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Bioassays

Benzon Research has performed thousands of potency bioassays on microbial pest control agents such as Bacillus thuringiensis, viruses, and fungi. Diet incorporation, diet surface contamination, and droplet feeding bioassays can be used with any of the lepidopteran species that are colonized in-house, along with other species available from outside sources. The picture to the right shows the scoring of a baculovirus bioassay against Helicoverpa zea.
Hzea bioassay reading


  Dosing mosquito larvae with B.t.i.
 Cups containing Aedes aegypti larvae (left) are being dosed with a production lot of B.t. israelensis for label standardization. We routinely test B.t.i. fermentation broths, concentrates,  powders, granules, briquets, and other formulations from all over the world.
Bioassays are also performed as part of fermentation or  formulation improvement efforts and storage stability studies.


The droplet-feeding bioassay works remarkably well for certain combinations of active ingredient and species. To the right, droplets containing blue dye and baculovirus particles are being presented to neonate larvae of the corn earworm, Helicoverpa zea . Fed larvae will be held on artificial diet for several days before determining mortality.
droplet bioassay

In addition to the above types of assays, Benzon Research can evaluate additives for feeding stimulation or deterency, perform topical applications of chemical insecticides to insects as small as mosquitoes, and evaluate sublethal effects.

 
See also:   repellents   household pests



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